The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively.

Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non- homologous end joining (NHEJ) required for double-strand break repair.

Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. PROTP12956 Ku-P70/P80 Ku P70/P80 Human Recombinant Protein KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W., Ford L.P., Lenertz L., Wright W.E., Shay J.W. Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors.

Ku70 uniprot

Ku70 antibody LS-C393647 is an unconjugated mouse monoclonal antibody to human Ku70 (XRCC6). Validated for ICC, IP and WB. 2.1. Identiﬁcation and Deletion of the KU70, HOG1 and MSB2 Homologs in A. oligospora The KU70 homologs of A. oligospora were identiﬁed in Blast2GO 5 Pro [23] by performing a local BLAST analysis. We used the amino acid sequences of Ku70 orthologs from S. cerevisiae (UniProt ID PLA0263 Sigma-Aldrich Rabbit anti-Ku70 Antibody, Affinity Purified Powered by Bethyl Laboratories, Inc. Synonym: 5′-dRP lyase Ku70, 5′-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, 70 kDa subunit, 70kDa, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, CTC box binding factor 75 kDa subunit, CTC box KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource. 2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase.

Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25915. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse samples. Supplied as 400 µL purified antibody (2 mg/mL).

Required for mating-type switching (By similarity). KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource. 2007-01-23 · Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

Polyclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Western Blotting Image 1: Ku70 (D35) Antibody UniProt ID: P12956.

ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource.

Uniprot: >>Visit The Human Protein Atlas. Gene ID: Gene Name: XRCC6. Molecular Weight: Observed Mol.Wt.: 70kD. Ku is a dimeric protein complex that binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.Ku is evolutionarily conserved from bacteria to humans.
Ccmt insert

Lane 1: HT1080 Whole Cell Lysate at 40ug.

Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse samples. Supplied as 400 µL purified antibody (2 mg/mL).
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KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement.

InterPro Ku70 has been referred to by several names including: Lupus Ku autoantigen protein p70; ATP-dependent DNA helicase 2 subunit 1; X-ray repair complementing X-ray repair cross-complementing protein 6 (KU70). Mol. type, Protein. Organism, Homo sapiens. Olig. state, Monomer. Mon. MW, 69.8 kDa. UniProt, P12956 X-ray repair complementing defective repair in Chinese hamster cells 6 .

The precise role of the Ku70 gene in this process has not yet been determined, in part because no cell lines, animals, or human diseases involved with deficiencies in this gene have yet been identified. Both the human and the mouse Ku70 cDNAs have been cloned, and the human gene has been mapped to chromosome 22q13.

a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86. The blocking of the Ku70 and Ku86 interaction by HSF1 induced defective NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in Ku70 or Ku80 Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples.

Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455).